Mobilization of energetic reserves during starvation in juveniles of different size of the redclaw crayfish Cherax quadricarinatus

CALVO N.S.; STUMPF L.; CORTES JACINTO E.; CASTILLO DÍAZ F; CALVO NS, LÓPEZ GRECO LS; LOPEZ GRECO LS; LOPEZ GRECO L.S

AQUACULTURE NUTRITION

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2018 vol. 24 p. 952 - 960

1353-5773

Mobilization patterns of energy reserves during starvation are highly variable amongcrustaceans, and understanding this process is useful to satisfy the nutritional requirementsof cultured organisms. The aim of this study was to elucidate the mobilizationpatterns on early and advanced juveniles?first free stage and one-gramjuveniles?ofredclaw crayfish (Cherax quadricarinatus). A biochemical approach was used to analysethe organic carbon, total proteins, lipids and glycogen levels of the entire animal, anda histochemical approach was adopted to identify location of metabolites in thehepatopancreas and abdominal muscle. While starvation did not affect early juvenileprotein levels, it showed a significant decrease in advanced juveniles. Histochemicalanalysis showed that lipid storage of hepatopancreas R cells was depleted near pointof no return (PNR) and recovered after feeding. Glycogen storage was localized in theF cells of the hepatopancreas, and among muscle fibres of the abdomen, where afterfeeding, a pronounced accumulation was observed. Early and advanced juveniles ofredclaw crayfish showed different patterns of consumption mainly related to the proteinlevel, which was the most abundant reserve in advanced juveniles and the mostconsumed during starvation. Hepatopancreas R cells were confirmed as the principallipid storage, whereas the abdominal muscle was the main glycogen storage. In crustaceanaquaculture, the understanding of resistance to temporary feed deprivation isrelevant to achieve an adequate feeding management, thereby avoiding the overfeedingand diminishing operating costs.