INVESTIGADORES
AGOSTINI Elizabeth
artículos
Título:
TOMATO (LYCOPERSICON ESCULENTUM CV. PERA) HAIRY ROOT CULTURES: CHARACTERIZATION AND CHANGES IN PEROXIDASE ACTIVITY UNDER NaCl TREATMENT
Autor/es:
MELINA A. TALANO; ELIZABETH AGOSTINI; MARIA I. MEDINA; SILVIA MILRAD DE FORCHETTI; HORACIO A. TIGIER
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Editorial:
SPRINGER
Referencias:
Lugar: Inglaterra; Año: 2003 vol. 39 p. 354 - 359
ISSN:
1054-5476
Resumen:
Hairy root cultures of Lycopersicon esculentum L. Mill cv. Pera were established by infection of leaf explants withLycopersicon esculentum L. Mill cv. Pera were established by infection of leaf explants with
Agrobacterium rhizogenes LBA 9402. The pattern of peroxidase isoenzymes in these tissues was similar to that of roots
excised from tomato plants grown in hydroponic cultures. Hairy root cultures may be an appropriate system to analyze the
peroxidase involvement in the response of isolated roots to salt stress, avoiding the problem of wounding or changes in
hormone levels observed in roots excised from plants. The cultures of hairy roots allowed the evaluation of changes in
peroxidase patterns not only in the tissue but also in the culture medium. Hairy roots were subcultured in Murashige and
Skoog liquid medium with or without 100mM NaCl to investigate the evolution of growth, total peroxidase activity of the
tissue and culture medium, and changes in the peroxidase isoenzyme patterns under each condition of growth. Control
cultures showed a growth index higher than those reported for other hairy root cultures, and it was even higher in the
presence of 100mM NaCl. The total peroxidase activity in the tissue was similar for control and salt-treated roots. Even
when the total peroxidase activity of the medium decreased under salt treatment, NaCl induced secretion of a highly basic
peroxidase and inhibition of the secretion of some acidic isoenzymes. These changes may explain the physiological role of
these enzymes in the response to salt stress that we will possibly establish through a future study of the biochemical
properties of those peroxidases.LBA 9402. The pattern of peroxidase isoenzymes in these tissues was similar to that of roots
excised from tomato plants grown in hydroponic cultures. Hairy root cultures may be an appropriate system to analyze the
peroxidase involvement in the response of isolated roots to salt stress, avoiding the problem of wounding or changes in
hormone levels observed in roots excised from plants. The cultures of hairy roots allowed the evaluation of changes in
peroxidase patterns not only in the tissue but also in the culture medium. Hairy roots were subcultured in Murashige and
Skoog liquid medium with or without 100mM NaCl to investigate the evolution of growth, total peroxidase activity of the
tissue and culture medium, and changes in the peroxidase isoenzyme patterns under each condition of growth. Control
cultures showed a growth index higher than those reported for other hairy root cultures, and it was even higher in the
presence of 100mM NaCl. The total peroxidase activity in the tissue was similar for control and salt-treated roots. Even
when the total peroxidase activity of the medium decreased under salt treatment, NaCl induced secretion of a highly basic
peroxidase and inhibition of the secretion of some acidic isoenzymes. These changes may explain the physiological role of
these enzymes in the response to salt stress that we will possibly establish through a future study of the biochemical
properties of those peroxidases.M NaCl to investigate the evolution of growth, total peroxidase activity of the
tissue and culture medium, and changes in the peroxidase isoenzyme patterns under each condition of growth. Control
cultures showed a growth index higher than those reported for other hairy root cultures, and it was even higher in the
presence of 100mM NaCl. The total peroxidase activity in the tissue was similar for control and salt-treated roots. Even
when the total peroxidase activity of the medium decreased under salt treatment, NaCl induced secretion of a highly basic
peroxidase and inhibition of the secretion of some acidic isoenzymes. These changes may explain the physiological role of
these enzymes in the response to salt stress that we will possibly establish through a future study of the biochemical
properties of those peroxidases.M NaCl. The total peroxidase activity in the tissue was similar for control and salt-treated roots. Even
when the total peroxidase activity of the medium decreased under salt treatment, NaCl induced secretion of a highly basic
peroxidase and inhibition of the secretion of some acidic isoenzymes. These changes may explain the physiological role of
these enzymes in the response to salt stress that we will possibly establish through a future study of the biochemical
properties of those peroxidases.