INVESTIGADORES
BOTTERO Daniela
artículos
Título:
Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection.
Autor/es:
BOTTERO D.; GAILLARD M.E.; AGUSTINA ERREA; GRISELDA MORENO; ZURITA E.; PIANCIOLA L.; MARTÍN RUMBO; D. HOZBOR
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2013 p. 5262 - 5268
ISSN:
0264-410X
Resumen:
Bordetella
parapertussis, a close related species of B. pertussis, can also cause the disease
named pertussis or whooping cough. The number of cases caused by this related
pathogen has risen sustained in the last years. The widely used cellular (wP)
or acellular (aP) pertussis vaccines have little or no efficacy against B. parapertussis. In an effort to devise
an effective acellular vaccine against B.
parapertussis infection, outer membrane vesicles (OMVs) were obtained from B. parapertussis. Proteomic analysis of
the resulting OMVs, designated OMVsBpp, evidenced the presence of several
surface immunogens including pertactin. The characterized OMVsBpp were used in
murine B. parapertussis intranasal
challenge model to examine their protective capacity when administered by
systemic route. Immunized BALB/c mice were challenged with sublethal doses of B. parapertussis. Significant
differences between immunized animals and the negative control group were
observed (p < 0.001). OMVsBpp protected against B. parapertussis infection, whereas current commercial aP vaccine showed
little protection against such pathogen. More interestingly, protection induced
by OMVsBpp against B. pertussis was comparable
to our previously designed vaccine consisting in OMVs derived from B. pertussis (OMVsBp). For these
experiments we used as a positive control the current commercial aP in high
dose. As expected aP offered protection against B. pertussis in mice. Altogether the results presented here showed
that the OMVs from B. parapertussis are
an attractive vaccine candidate to protect against whooping cough induced by B. parapertussis but also by B. pertussis.