Effect of GnRH agonist and antagonist on human luteinized granulosa cells proliferation and apoptosis

VITALE ALEJANDRA; ABRAMOVICH DALHIA; PELUFFO MARINA; MERESMAN GABRIELA; TESONE MARTA

FERTILITY AND STERILITY

Elsevier Science Inc.

Año: 2006 vol. 85 p. 1064 - 1067

0015-0282

The GnRH agonist leuprolide acetate (LA) inhibited DNA synthesis in epidermal growth factor–stimulated human granulosa luteinized cell cultures. This effect was blocked by the prior addition of a GnRH antagonist antide (ANT), and this compound per se was able to produce a stimulatory effect of DNA synthesis on basal conditions. Leuprolide acetate produced an increase in the percentage of apoptotic cells, and when these two factors were co-incubated, ANT blocked the apoptotic effect produced by LA. (Fertil Steril
2006; 85:1064 –7. ©2006 by American Society for Reproductive Medicine.) 85:1064 –7. ©2006 by American Society for Reproductive Medicine.) 85:1064 –7. ©2006 by American Society for Reproductive Medicine.) 85:1064 –7. ©2006 by American Society for Reproductive Medicine.) 85:1064 –7. ©2006 by American Society for Reproductive Medicine.)
2006; 85:1064 –7. ©2006 by American Society for Reproductive Medicine.)