Burkholderia puraquae sp. nov., a novel Burkholderia cepacia complex species from hospital setting samples

P. MARTINA,; A. BOSCH

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.

SOC GENERAL MICROBIOLOGY

Lugar: London; Año: 2018 vol. 68 p. 14 - 20

1466-5026

During a 12 years of epidemiological surveillanceinvolving Burkholderia cepaciacomplex (Bcc) bacteria from respiratory secretions of local patients withcystic fibrosis and hospitals settings, we found six Bcc isolates whose concatenated allele sequences diverged by more than3% with the yet described Bcc-species. These isolates together with an additional Bcc isolate, PBP 78 (=R-50214) obtainedfrom the Bukholderia PubMLST database,grouped in the same multi-locus sequence analysis-based cluster within the Bcc.Therefore, we examined the sixisolates by a polyphasic approach, which included recA, 16S rRNA and hisA sequence analysis, multilocus sequence analysis (MLSA), DNAbase composition, fatty acid metyl ester analysis, RAPD genotyping, MALDITOF-MSspectrometry, and biochemical identification by classical and automated systemtests. Among the isolates of thenovel taxon the concatenated allele sequence divergence was 1.8± 0.1%, and theaverage divergence towards their nearest neighbour, B. metallica was 4.3 ±0.2%, and towards their farest neighbour B. multivorans was 7.3±0.1%. Based onthe obtained results, we could confirm that the 7 isolates represent a singlenovel species within the Bcc, for which we propose the name Burkholderia puraquae sp nov. The typestrain is B. puraquae CAMPA1040T which can be differentiated from other Bcc species by recA gene sequence analysis, MLSA andMALDI-TOF analysis, and only few biochemical tests such as growth at 42 ºC, lysine decarboxylaseand β-galactosidase activity and esculin hydrolysis.