Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses

FIORENTINO, MARÍA ANDREA; PAOLICCHI, FERNANDO ALBERTO; CAMPERO, CARLOS MANUEL; BARBEITO, CLAUDIO G.

Open Veterinary Journal

Ajol

Lugar: Tripoli; Año: 2018 vol. 8 p. 57 - 63

2226-4485

Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates theidentification of complex structures to which the terminal sugar attaches and may reveal physiological or pathologicalchanges in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigatethe effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovineplacentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 wereexamined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigenswere immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA,PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA wasfound in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharidedistribution in the lungs and placenta of B. abortus-infected fetuses.