45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin-starch films: evaluation of antibacterial and cytotoxic effects

RIVADENEIRA, J.; DI VIRGILIO, A; AUDISIO, M. C.; BOCCACCINI, AR; GORUSTOVICH, A.A.

JOURNAL OF MATERIALS SCIENCE

SPRINGER

Lugar: Berlin; Año: 2017 p. 9091 - 9102

0022-2461

The aim of this work was to evaluate the release profile of vancomycin hydrochloride(VC), as well as the degradation, in vitro antistaphylococcal effect and cytotoxicity inMG-63 osteoblast-like cells of gelatin-starch (GS) films added with differentconcentrations of microparticles of the bioactive glass 45S5 (m-BG). The biomaterialswere obtained through the gel casting method. Four different composites wereprepared at four different weight percentages of m-BG: 0%, 5%, 10% and 15%.Glutaraldehyde 0.25 wt% (GA) was used as the cross-linker. The composites werecharacterized by scanning electron microscopy and the in vitro degradation of the filmswas studied by measuring the water uptake and weight loss. The drug release kineticswas quantified spectrophotometrically. The inhibition zone test and the plate countmethod were used to evaluate the antibacterial activity of the samples. Threestaphylococcus strains were evaluated: Staphylococcus aureus ATCC6538,Staphylococcus aureus ATCC29213, and Staphylococcus epidermidis ATCC12228.Cytotoxicity effects were evaluated through the MTT assay. The addition of m-BG toGS films showed no effects on the amount of water uptake, but led to an increase inthe weight loss over time, even with m-BG content. The release rate of VC was alsoaffected by the increasing concentration of m-BG in the composite films. However, theantibacterial effects of the composites were not improved by this modulation. Allcomposites strongly inhibited staphylococcal cells with similar strength. On the otherhand, liquid extracts from the composites resulted in cytotoxic effects on MG-63osteoblast-like cells due to the presence of GA, but not to the concentration of VC orm-BG.