INVESTIGADORES
IBARRA cristina Adriana
artículos
Título:
Intracerebroventricular administration of Shiga toxin type 2 induces striatal neuronal death and glial alterations: an ultraestructural study
Autor/es:
GOLDSTEIN, J, LOIDL CF, PISTONE CREYDT V, BOCCOLI J, IBARRA C
Revista:
BRAIN RESEARCH
Editorial:
Elsevier
Referencias:
Lugar: Oxford; Año: 2007 vol. 1161 p. 106 - 115
ISSN:
0006-8993
Resumen:
Abstract
Shiga toxin (Stx) from enterohemorrhagic Escherichia coli (STEC) is the main cause of
hemorrhagic colitis which may derive to hemolyticuremic syndrome (HUS). HUS is
characterized by acute renal failure, thrombocytopenia and microangiopathic hemolytic
anemia. Mortality in the acute stage has been lower than 5% of total affected argentine
children with endemic HUS. Common signs of severe CNS involvement leading to death
included seizures, alteration of consciousness, hemiparesis, visual disturbances, and
brainstem symptoms. The main purpose of the present work was to study the direct
involvement of Stx2 in brain cells by intracerebroventricular (i.c.v.) administration of Stx2.
Immunodetection of Stx2 was confirmed by immunoelectron cytochemistry in different
subsets and compartments of affected caudate putamen cells of corpus striatum.
Transmission electron microscopy (TEM) studies revealed apoptotic neurons, glial
ultrastructural alterations and demyelinated fibers. The i.c.v. microinfusion was applied
for the first time in rats to demonstrate the direct action of Stx2 in neurons and glial cells. The
toxin may affect brain neuroglial cells without the involvement of proinflammatory or
systemic neurotoxic elements.