INVESTIGADORES
IBARRA cristina Adriana
artículos
Título:
Cytotoxic effect of Shiga toxin-2 holotoxin and its B subunit on human renal tubular epithelial cells.
Autor/es:
PISTONE CREYDT V, SILBERSTEIN C, ZOTTA E, IBARRA C
Revista:
MICROBES AND INFECTION
Editorial:
Elsevier
Referencias:
Lugar: Paris; Año: 2006 vol. 8 p. 410 - 419
ISSN:
1286-4579
Resumen:
Abstract
Shiga toxin-producing Escherichia coli produces watery diarrhea, hemorrhagic colitis and hemolyticuremic syndrome (HUS). In Argen-
tina, HUS is the most common cause of acute renal failure in children. The purpose of the present study was to examine the cytotoxicity of Stx
type 2 (Stx2 holotoxin) and its B subunit (Stx2 B subunit) on human renal tubular epithelial cells (HRTEC), in the presence and absence of
inflammatory factors. Cell morphology, cell viability, protein synthesis and apoptosis were measured. HRTEC are sensitive to both Stx2 holo-
toxin and Stx2 B subunit in a dose- and time-dependent manner. IL-1, LPS and butyrate but not TNF, IL-6 and IL-8, increased the Stx
mediated cytotoxicity. The effects of Stx2 B subunit appear at doses higher than those used for Stx2 holotoxin. Although the physiological
importance of these effects is not clear, it is important to be aware of any potentially toxic activity in the B subunit, given that it has been
proposed for use in a vaccine.