Probiotic activity of Enterococcus faecalis CECT7121: effects on mucosal immunity and intestinal epithelial cells

CASTRO MS; MOLINA, MA; AZPIROZ, MB; DÍAZ AM; PONZIO, R; SPARO, MÓNICA; MANGHI MA; CANELLADA, A

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2016 vol. 121 p. 1117 - 1129

1364-5072

AbstractAims: To analyze the effect of Enterococcus faecalis CECT7121 on intestinal epithelial cells and its effects on the mucosal immune response.Methods and Results: Ent. faecalis CECT7121 showed a high adhesion capacity to completely and heterogeneously differentiated human intestinal epithelial cell line (Caco-2 cells). In addition, the contact of this bacterium with Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA+ and IL-6+ cells in the small intestine, as well as the production of inflammatory cytokines (TNFα, IL-6, and IL-12) in the gut, was determined after intragastric inoculation of Ent. faecalis CECT7121 in BALB/c mice. The administration of Ent. faecalis CECT7121 increased the number of IgA+ cells in the intestinal lamina propria without modifying the percentage of IL-6+ cells. No differences were observed in the cytokines measured in the intestinal extracts between probiotic-treated and control mice.Conclusions: Ent. faecalis CECT7121 stimulates local mucosal immunity and adheres to intestinal epithelial cells without inducing inflammatory signals. Significance and Impact of the Study: Our results indicate that, apart from its already reported systemic immune activity, Ent. faecalis CECT7121 has a modulatory effect at a local level.