Dopamine agonists upregulate IL-6 and IL-8 production in human keratinocytes.

PARRADO, CECILIA; CANELLADA, ANDREA; GENTILE, MARÍA TERESA; REY-ROLDÁN, ESTELA

NEUROIMMUNOMODULATION.

KARGER

Año: 2012 vol. 19 p. 359 - 366

1021-7401

Aim: Catecholamines regulate functions of the nervous, neuroendocrine and immune systems. Dopamine may modulate the activity of keratinocytes, which play a role by secreting cytokines and chemokines.The aim of this study was to evaluate the effect of dopaminergic agonists on the production of IL-6 and IL-8 by a non-tumoral human keratinocyte cell line (HaCaT).  Methods: Cells were stimulated with dopamine and the D2 dopamine receptor agonist cabergoline. Levels of IL-6 and IL-8 in culture supernatants were then determined. Cell proliferation was measured also assessed. Assays were carried out in the presence or absence of the dopaminergic and ß-adrenergic receptors antagonists (sulpiride and propranolol, respectively) and ascorbic acid.   Results: Dopamine stimulated the production of IL-6 and IL-8 in a concentration-dependent manner. The effects observed on the secretion of IL-6 were more potent than those corresponding to IL-8 and were reduced by ascorbic acid. The dopamine-induced IL-6 secretion was partially reduced by sulpiride and abrogated by propranolol. The latter drug was able to block the effect of dopamine on the secretion of IL-8. The cabergoline-induced IL-6 release was reduced by sulpiride. Cell viability was not affected by any of the drugs.  Conclusions: Dopaminergic agonists can stimulate keratinocytes to produce IL-6 and IL-8 which are related to inflammatory cutaneous processes. These effects would be mediated by dopaminergic and β-adrenergic receptors and by receptor-independent oxidative mechanisms.