INVESTIGADORES
GUDESBLAT Gustavo Eduardo
artículos
Título:
ANALYSIS OF AN ABSCISIC ACID (ABA)-RESPONSIVE GENE PROMOTER BELONGING TO THE ASR GENE FAMILY FROM TOMATO IN HOMOLOGOUS AND HETEROLOGOUS SYSTEMS
Autor/es:
MAGDALENA ROSSI; FERNANDO CARRARI; JOSÉ LUIS CABRERA-PONCE; CECILIA VAZQUEZ-ROVERE; LUIS HERRERA-ESTRELLA; GUSTAVO EDUARDO GUDESBLAT; NORBERTO DANIEL IUSEM
Revista:
MOLECULAR AND GENERAL GENETICS
Editorial:
Springer
Referencias:
Año: 1998 vol. 258 p. 1 - 8
ISSN:
0026-8925
Resumen:
Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a
recently reported member of this family, is believed to be regulated by
abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been
fully sequenced, and candidate upstream regulatory elements have been
identified. To prove that the promoter region is functional in vivo, we
fused it upstream of the beta-glucuronidase (GUS) reporter gene. The
resulting chimeric gene fusion was used for transient expression assays
in papaya embryogenic calli and leaves. In addition, the same construct
was used to produce transgenic tomato, papaya, tobacco, and potato
plants. Asr2 upstream sequences showed promoter function in all of these
systems. Under the experimental conditions tested, ABA stimulated GUS
expression in papaya and tobacco, but not in tomato and potato systems.