Screening of lipases with unusual high activity in esterification of sn-2 position of 1,3-dicaprin under mild operating conditions

SANCHEZ D.; TONETTO G.; FERREIRA M.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2017 vol. 65 p. 5010 - 5017

0021-8561

In this work, the synthesis ofacylglycerides with high nutritional value was carried out by enzymaticesterification at sn-2 position of 1,3-dicaprin with palmitic acid. Acomparative study of the performance of several biocatalysts according to the obtainedproducts was carried out. The results obtained with several of the biocatalystsevaluated are very interesting, and it would be possible to use them to obtaina mixture of acylglycerides to act as a fat substitute. The final product wascomposed of about 90% of nutritionally attractive glycerides. These glycerideswere medium-chain length triglycerides, medium−long chain triglycerides (mainlytriglycerides with medium chain fatty acids at sn-1 and sn-3 positions and longchain fatty acid at sn-2 position), and 1,3-diglycerides. Pseudomonas fluorescens lipase and Burkholderia cepacia lipase immobilized on chitosan demonstratedunusual high activity in the sn-2 esterification of 1,3-dicaprin with palmiticacid at 45 °C and 12 h with 33% yield to 1,3-dicaproyl-2-palmitoyl glycerol.Burkholderia cepacia lipase has the advantage of being immobilized; however,BCL/chitosan has the advantages of being immobilized and therefore its easyrecovery from the reaction media.