INVESTIGADORES
SALOMON Oscar Daniel
artículos
Título:
General, biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
Autor/es:
DE ROODT AR; CORONAS FI; LAGO NR; GONZALEZ ME; LASKOWICZ RD; BELTRAMINO JC; SAAVEDRA S; LOPEZ R; REATI G; VURCHARCHUK MG; BAZAN E; VARNI L; SALOMON OD; POSSANI LD
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 vol. 55 p. 307 - 319
ISSN:
0041-0101
Resumen:
fatalities in the country, however no systematic studies have been conducted with the
venom of this species. This communication describes a general biochemical and immunological
characterization of the venom obtained from T. trivittatus scorpions collected in
the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre
Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents
were reported to occur due to this scorpion. For comparative purposes two types of
samples were assayed: whole soluble venom obtained by electrical stimulation and
supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1)
were used for LD50 determinations by two distinct routes of administration (intravenously
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre
Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents
were reported to occur due to this scorpion. For comparative purposes two types of
samples were assayed: whole soluble venom obtained by electrical stimulation and
supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1)
were used for LD50 determinations by two distinct routes of administration (intravenously
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
T. trivittatus scorpions collected in
the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre
Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents
were reported to occur due to this scorpion. For comparative purposes two types of
samples were assayed: whole soluble venom obtained by electrical stimulation and
supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1)
were used for LD50 determinations by two distinct routes of administration (intravenously
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
50 determinations by two distinct routes of administration (intravenously
and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg
mouse body weight. Samples of soluble venom were always more potent than Telson
homogenates. More complex venom was observed in homogenates compared to soluble
venom, as expected. This was supported by gel electrophoretic analysis and high performance
liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
(HPLC) separations. Additionally, the HPLC profile was
enriched in proteins resolved at similar elution times as other known toxins from scorpion
venoms studied. Immune enzymatic assays were also conducted comparatively, using four
different anti-venoms commercially available for treatment of scorpion stings (Argentinean
antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn
from the Mexican Bioclon Institute). Cross-reactivities were observed and are
reported among the various venoms and anti-venoms used. Lung, heart and pancreas
pathological modifications were observed on tissues of intoxicated mice. It seems that
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.
there are important variations on the venom compositions of the various samples studied
and reported here, depending on the geographical area where the scorpions were
captured. The results reported here are important for the clinical outcome of human
accidents.