Multiway calibration strategy with chromatographic data exploiting the second-order advantage for quantitation of three antidiabetic and three antihypertensive drugs in serum samples

MONZON C; TEGLIA C; DELFINO M; GOICOECHEA H C

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 136 p. 185 - 192

0026-265X

This paper proposes a multiway calibration strategy implementing the modeling with MCR-ALS and U-PLS/RBL of second-order chromatographic data for quantitation of six analytes: gliclazide, glibenclamide, glimepiride, atenolol, enalapriland amlodipine in serum samples, in an analysis time of 3 min.The performance of both algorithms was compared in terms of predictive ability, showing relative error of predictionvalues below 10% in all cases. LOD values calculated are below 30 ng mL− 1 for all the studied drugs, which allowdetection in human serum in patients under treatment. U-PLS/RBL has higher sensitivity and better detection and quantificationlimits for all the studied analytes; however results obtained by MCR-ALS enable its usage as well. Both methodsprovide comparable results for glibenclamide, glimepiride and gliclazide. With this multiway calibration strategy, thepresence of enalapril, amlodipine and atenolol could be quantitated with high accuracy. Run time was reduced by 50%considering previous reports, as well as reduction of solvents, in accordance with green chemistry principles.