INVESTIGADORES
ISLA Maria Ines
artículos
Título:
Production of plant protoplasts by enzymes from selected wood-destroying fungi
Autor/es:
D. P. LEAL*, M. I. ISLA**, M. A. VATTUONE**, A. R. SAMPIETRO**
Revista:
Applied Microbiology and Biotechnology
Editorial:
Springer-Verlag
Referencias:
Año: 1994 vol. 41 p. 58 - 61
Resumen:
The goal of this work was to select higher
fungi for the production of enzymes able to produce
plant protoplasts. The hydrolytic capacity of various
species of wood-destroying fungi was examined to select
those with the maximal hydrolytic capacity. The
selection criteria included attack on cellulose, and oligo-
and polysaccharides by each fungus species. Lenzites
elegans, Ganoderma applanatum and Pycnoporus
sanguineus produced the richest enzymic mixtures,
which were evaluated for their protoplasting ability. L.Lenzites
elegans, Ganoderma applanatum and Pycnoporus
sanguineus produced the richest enzymic mixtures,
which were evaluated for their protoplasting ability. L.and Pycnoporus
sanguineus produced the richest enzymic mixtures,
which were evaluated for their protoplasting ability. L.produced the richest enzymic mixtures,
which were evaluated for their protoplasting ability. L.
elegans has the best enzymic mixture able to produce
plant protoplasts with the shortest time for maceration
of plant tissues, followed by G. applanatum and P. sanguineus.has the best enzymic mixture able to produce
plant protoplasts with the shortest time for maceration
of plant tissues, followed by G. applanatum and P. sanguineus.G. applanatum and P. sanguineus.
The potential of these fungi and the simplicity
of enzyme production suggest the possibility of commercial
applications