INVESTIGADORES
PINONI Silvina Andrea
artículos
Título:
Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
Autor/es:
PINONI, S. A. Y LÓPEZ MAÑANES, A. A.
Revista:
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
Editorial:
Elsevier
Referencias:
Año: 2009 p. 91 - 97
ISSN:
0022-0981
Resumen:
The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to
environmental salinity of the coexistent Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities were
studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita
coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na++ ATPase activity and the response to
environmental salinity of the coexistent Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities were
studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita
coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+environmental salinity of the coexistent Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities were
studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita
coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+Neohelice (Chasmagnathus) granulata from Mar Chiquita
coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na++
ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive,
furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemideinsensitive
and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed
MichaelisMenten kinetics (Km=0.021 and 0.224 mM, respectively). These characteristics appeared to be
quite different from those previously described for Na+K+ ATPase activity in chela muscle of this crab. Na+K+I50 for ouabain-insensitive,
furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemideinsensitive
and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed
MichaelisMenten kinetics (Km=0.021 and 0.224 mM, respectively). These characteristics appeared to be
quite different from those previously described for Na+K+ ATPase activity in chela muscle of this crab. Na+K++ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemideinsensitive
and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed
MichaelisMenten kinetics (Km=0.021 and 0.224 mM, respectively). These characteristics appeared to be
quite different from those previously described for Na+K+ ATPase activity in chela muscle of this crab. Na+K++ ATPase activities were weakly affected by pH and showed
MichaelisMenten kinetics (Km=0.021 and 0.224 mM, respectively). These characteristics appeared to be
quite different from those previously described for Na+K+ ATPase activity in chela muscle of this crab. Na+K+Menten kinetics (Km=0.021 and 0.224 mM, respectively). These characteristics appeared to be
quite different from those previously described for Na+K+ ATPase activity in chela muscle of this crab. Na+K++K+ ATPase activity in chela muscle of this crab. Na+K+
ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to
environmental salinity. In crabs acclimated to low salinity (10), a salinity at which N. granulata exhibits a
strong hyperregulatory capacity, Na+K+ ATPase activitywas higher (117±26 nmol Pi min−1 mg prot−1) than in
35 salinity (23±6 nmol Pi min−1 mg prot−1) (a salinity at which this crab is osmoionoconforming). On the
contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+ ATPase activities appeared to be sensitive to
environmental salinity. In crabs acclimated to low salinity (10), a salinity at which N. granulata exhibits a
strong hyperregulatory capacity, Na+K+ ATPase activitywas higher (117±26 nmol Pi min−1 mg prot−1) than in
35 salinity (23±6 nmol Pi min−1 mg prot−1) (a salinity at which this crab is osmoionoconforming). On the
contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.), a salinity at which N. granulata exhibits a
strong hyperregulatory capacity, Na+K+ ATPase activitywas higher (117±26 nmol Pi min−1 mg prot−1) than in
35 salinity (23±6 nmol Pi min−1 mg prot−1) (a salinity at which this crab is osmoionoconforming). On the
contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+K+ ATPase activitywas higher (117±26 nmol Pi min−1 mg prot−1) than in
35 salinity (23±6 nmol Pi min−1 mg prot−1) (a salinity at which this crab is osmoionoconforming). On the
contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed. salinity (23±6 nmol Pi min−1 mg prot−1) (a salinity at which this crab is osmoionoconforming). On the
contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+ ATPase activity was higher in 35 salinity (108±
15 nmol Pi min−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.−1 mg prot−1) than in crabs acclimated to 10 salinity (36±11 nmol Pi min−1 mg prot−1).
Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+ ATPase activity was not affected by acclimation of crabs to
low salinity. The response to low salinity suggests that Na+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+K+ ATPase could be a component of muscle
regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive,
furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible
differential functional roles of Na+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.+K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are
discussed.