INVESTIGADORES
ALVAREZ Gladis Susana
artículos
Título:
Resistance of Young Mice to Pneumococcal Infection can be Improved by Oral Vaccination with Recombinant Lactococcus lactis
Autor/es:
VILLENA JULIO; MEDINA MARCELA; RACEDO SILVIA; ALVAREZ SUSANA
Revista:
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
Editorial:
SCIENTIFIC COMMUNICATIONS INT LTD
Referencias:
Año: 2010 vol. 43 p. 1 - 10
ISSN:
1684-1182
Resumen:
BACKGROUND/PURPOSE: Oral immunization with Lactococcus lactis PppA (LPA +), a recombinant
strain that is able to express the pneumococcal protective protein A, can improve the resistance to respiratory
challenge with Streptococcus pneumoniae in adult mice. In this study, we investigated whether oral immunization
protocols using LPA+ are able to protect young mice against pneumococcal respiratory infection.Oral immunization with Lactococcus lactis PppA (LPA +), a recombinant
strain that is able to express the pneumococcal protective protein A, can improve the resistance to respiratory
challenge with Streptococcus pneumoniae in adult mice. In this study, we investigated whether oral immunization
protocols using LPA+ are able to protect young mice against pneumococcal respiratory infection.Streptococcus pneumoniae in adult mice. In this study, we investigated whether oral immunization
protocols using LPA+ are able to protect young mice against pneumococcal respiratory infection.+ are able to protect young mice against pneumococcal respiratory infection.
METHODS: Young mice (aged, 3 weeks) were immunized orally with LPA+ for 5 consecutive days.
Vaccination was performed once (non-boosted group), or twice with a 2-week interval between each immunization
(boosted group). At the end of treatment, the specific immune responses and the resistance to
pneumococcal infection were studied.Young mice (aged, 3 weeks) were immunized orally with LPA+ for 5 consecutive days.
Vaccination was performed once (non-boosted group), or twice with a 2-week interval between each immunization
(boosted group). At the end of treatment, the specific immune responses and the resistance to
pneumococcal infection were studied.
RESULTS: We found that the oral immunization with LPA+ was able to induce the production of specific
antibodies in the respiratory and intestinal tracts as well as systemically. Analysis of IgG subtypes showed that
LPA+ immunization stimulated a mixed Th1 and Th2 response. To assess whether the production of mucosal
and systemic antibodies was able to afford protection against respiratory pneumococcal infection, challenge
experiments with the pathogenic serotypes 3, 6B, 14, and 23F were carried out. Vaccination with LPA+ was
able to increase resistance to infection with the four serotypes of S. pneumoniae, although the protective
capacity of the experimental vaccine was different for each of them. Immunization decreased colonization in
the lung, prevented bacteremia of serotypes 6B, 14, and 23F, and decreased colony counts of serotype 3.We found that the oral immunization with LPA+ was able to induce the production of specific
antibodies in the respiratory and intestinal tracts as well as systemically. Analysis of IgG subtypes showed that
LPA+ immunization stimulated a mixed Th1 and Th2 response. To assess whether the production of mucosal
and systemic antibodies was able to afford protection against respiratory pneumococcal infection, challenge
experiments with the pathogenic serotypes 3, 6B, 14, and 23F were carried out. Vaccination with LPA+ was
able to increase resistance to infection with the four serotypes of S. pneumoniae, although the protective
capacity of the experimental vaccine was different for each of them. Immunization decreased colonization in
the lung, prevented bacteremia of serotypes 6B, 14, and 23F, and decreased colony counts of serotype 3.+ immunization stimulated a mixed Th1 and Th2 response. To assess whether the production of mucosal
and systemic antibodies was able to afford protection against respiratory pneumococcal infection, challenge
experiments with the pathogenic serotypes 3, 6B, 14, and 23F were carried out. Vaccination with LPA+ was
able to increase resistance to infection with the four serotypes of S. pneumoniae, although the protective
capacity of the experimental vaccine was different for each of them. Immunization decreased colonization in
the lung, prevented bacteremia of serotypes 6B, 14, and 23F, and decreased colony counts of serotype 3.+ was
able to increase resistance to infection with the four serotypes of S. pneumoniae, although the protective
capacity of the experimental vaccine was different for each of them. Immunization decreased colonization in
the lung, prevented bacteremia of serotypes 6B, 14, and 23F, and decreased colony counts of serotype 3.S. pneumoniae, although the protective
capacity of the experimental vaccine was different for each of them. Immunization decreased colonization in
the lung, prevented bacteremia of serotypes 6B, 14, and 23F, and decreased colony counts of serotype 3.
CONCLUSION: We have shown that the oral immunization of young mice with LPA+ effectively induces
the production of specific antibodies against the antigen PppA, both in mucosae and at the systemic level.We have shown that the oral immunization of young mice with LPA+ effectively induces
the production of specific antibodies against the antigen PppA, both in mucosae and at the systemic level.