BECAS
NICOLA CANDIA Alejandro Javier
artículos
Título:
Oncolytic Adenovirus-Loaded Menstrual Blood Stem Cells Overcome the Blockade of Viral Activity Exerted by Ovarian Cancer Ascites
Autor/es:
ALFANO, ANA LAURA; NICOLA CANDIA, ALEJANDRO; CUNEO, NICASIO; GUTTLEIN, LEANDRO N.; SODERINI, ALEJANDRO; ROTONDARO, CECILIA; SGANGA, LEONARDO; PODHAJCER, OSVALDO L.; LOPEZ, M. VERONICA
Revista:
Molecular Therapy - Oncolytics
Editorial:
Molecular therapy
Referencias:
Año: 2017 vol. 6 p. 31 - 44
ISSN:
2372-7705
Resumen:
Patients with ovarian cancer present peritoneal ascites at recur-rence as a marker of disseminated disease and dismal prog-nosis. Oncolytic immunotherapy is an emerging approach forthe treatment of disseminated cancer. In the present work,we constructed a novel oncolytic adenovirus, AR2011, to targetmalignant ovarian tumors. AR2011 exhibited a clear lytic effectin vitro in human ovarian cancer cell lines and malignant cellsobtained from asciticfluids (AFs) of patients with ovarian can-cer. AR2011 activity was neutralized by antibodies present in31 samples of patient-derived AFs. However, this blockadewas overridden by preloading menstrual blood stem cells(MenSCs) with AR2011 (MenSC-AR), since AFs exerted noin vitro inhibitory effect on viral lytic activity under these con-ditions. Moreover, soluble factors present in AFs act as MenSCchemoattractants. MenSC-AR treatment of nude mice carryingestablished peritoneal carcinomatosis following administra-tion of human ovarian cancer cells was able to inhibit tumorgrowth at levels similar to those observed with AR2011 alone.This study demonstrates that MenSCs can be used to overridethe blockade that AFs exert on viral oncolytic effects.
