INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
High expression level of a foot and mouth disease virus epitope in tobacco transplastomic plants
Autor/es:
EZEQUIEL MATÍAS LENTZ, MARÍA EUGENIA SEGRETIN, MAURO MIGUEL MORGENFELD, SONIA ALEJANDRA WIRTH, MARÍA JOSÉ DUS SANTOS, MARINA VALERIA MOZGOVOJ, ANDRÉS WIGDOROVITZ, FERNANDO FÉLIX BRAVO-ALMONACID
Revista:
PLANTA
Editorial:
Springer
Referencias:
Año: 2009
ISSN:
0032-0935
Resumen:
Chloroplast transformation has an extraordinary
potential for antigen production in plants because of
the capacity to accumulate high levels of recombinant proteins
and increased biosafety due to maternal plastid inheritance
in most crops. In this article, we evaluate tobacco
chloroplasts transformation for the production of a highly
immunogenic epitope containing amino acid residues 135
160 of the structural protein VP1 of the foot and mouth disease
virus (FMDV). To increase the accumulation levels,
the peptide was expressed as a fusion protein with the
-glucuronidase reporter gene (uidA). The recombinant
protein represented the 51% of the total soluble proteins in
mature leaves, a level higher than those of the Rubisco
large subunit, the most abundant protein in the leaf of a
wild-type plant. Despite this high accumulation of heterologous
protein, the transplastomic plants and wild-type
tobacco were phenotypically indistinguishable. The FMDV
epitope expressed in transplastomic plants was immunogenic
in mice. These results show that transplastomic
tobacco express eYciently the recombinant protein, and we
conclude that this technology allows the production of large
quantities of immunogenic proteins.