Integration of Biorecognition Elements on PEDOT Platforms through Supramolecular Interactions

SAPPIA, LUCIANO D.; PICCININI, ESTEBAN; MARMISOLLÉ, WALDEMAR; SANTILLI, NATALIA; MAZA, ELIANA; MOYA, SERGIO; BATTAGLINI, FERNANDO; MADRID, ROSSANA E.; AZZARONI, OMAR

Advanced Materials Interfaces

WILEY-VCH Verlag GmbH & Co.

Lugar: Weinheim; Año: 2017

The rapidly emerging field of organic bioelectronics exploits the functional versatility of conducting polymers to transduce biological recognition events into electronic signals. For the majority of biosensors or biomedical devices, immobilization of a biorecognition element is a critical step to improve the biotic/abiotic interface. In this work, we describe a simple strategy to construct large-area all-plastic poly(3,4-ethylenedioxythiophene) (PEDOT) electrodes displaying carbohydrate biorecognizable motifs. First, the method involves the preparation of poly(allylamine)-PEDOT composites through supramolecular interactions. We demonstrated by Raman and X-ray spectroscopy (XPS) and cyclic voltammetry (CV) that the PEDOT:PAH ratio and the film electoactivity can be easily controlled by varying the amount of PAH in the PEDOT polymerization solution. Then, carbohydrate motifs were covalently anchored to the primary amine groups by a straightforward route using divinylsulfone chemistry. The recognition-driven assembly of the lectin cancanavalin A (Con A) and the glycoenzyme glucose oxidase (GOx) onto mannosylated surfaces was demonstrated by surface plasmon resonance (SPR) spectroscopy. Furthermore, the bioelectrocatalytic detection glucose through the assembled redox enzyme was studied for the all-plastic electrodes and compared to gold electrodes. Interestingly, the synergistic combination of conducting polymers and recognition-directed assembly led to a 2.7-fold enhancement of the bioelectrocatalitic signal. The enzyme surface coverage on the PEDOT platforms was adjusted using the layer-by-layer technique. Even though these devices were constructed on thin Plexiglas sheets, the obtained sensing capabilities are comparable to those from other PEDOT-based glucose sensors prepared on metallic substrates.