PERSONAL DE APOYO
OTAROLA Jessica Johana
artículos
Título:
Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam
Autor/es:
JESSICA OTAROLA; ADRIANA GUILLERMINA LISTA; BEATRIZ FERNÁNDEZ BAND; MARIANO GARRIDO
Revista:
Journal of Pharmaceutical Analysis
Editorial:
Elsevier
Referencias:
Año: 2014 vol. 5 p. 70 - 73
ISSN:
2347-2340
Resumen:
A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers (NLCs). The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the pH of the electrophoretic buffer solution and the applied voltage. The piroxicam peak was obtained with a satisfactory resolution. The separation was carried out using a running buffer composed of 50 mM ammonium acetate and 13.75 mM ammonia at pH 9. The optimal voltage was 20 kV and the cartridge temperature was 20 1C. The corresponding calibration curve was linear over the range of 2.7?5.4 mg/mL of NLC suspension. The reproducibility of migration time and peak area were investigated, and the obtained RSD% values (n¼5) were 0.99 and 2.13, respectively.