DEHYDROFUKINONE, MAIN METABOLITE FROM Senecio punae IMPROVES CIPROFLOXACIN ACTIVITY AND STIMULATES LACTOBACILLI BIOFILMS

VERNI C.; GARAY A.; BARDON A.; BORKOSKY S.; CARTAGENA E.

BIOCELL

XXXI ANUAL SCIENTIFIC MEETING

Año: 2014 vol. 38 p. 103 - 103

A pale yellow oil identified as dehydrofukinone (D) was isolated from the aerial parts of S. punae with a high yield (1.7%) and purity. We present here the results of the synergic effects of different ciprofloxacin and D mixtures against Gram (+) y () bacteria. The amount of biofilm formation and the antibacterial effects of the mixtures were evaluated with micro-dilution method. We employed four pathogenic strains: Pseudomonas aeruginosa ATCC 27853, P. aeruginosa isolated from skin injuries, Staphylococcus aureus ATCC 6538 P and an isolate methicillin resistant strain (MRSA) from clinical specimen. Probiotic bacteria were Lactobacillus acidophilus ATCC 521, L. casei ssp. paracasei C2 and L. plantarum CE 105. The tensioactive activity of non pathogenic strains was also evaluated employing the ?Oil Spreading? technique. Compound D has weak antibacterial and antibiofilm effects against both strains of P. aeruginosa but a mixture of D (23 µg/ml) and C (0.25 µg/ml) displays a strong synergic effect for both strains (FIC = 0.38) and reduces significantly the biofilm formation compared to control. S. aureus ATCC and MRSA showed MIC values of 3.00 and 1.50 mg/ml, respectively. Sub-lethal doses of D (50 and 25 µg/ml) also diminish significantly S. aureus biofilms (87.46 and 68.12%, respectively). On the other hand, D at 23 µg/ml improved the antibiotic action of C (FIC = 0.25), and the inhibition of biofilm. The results show that different associations of D and C have no antibacterial effects against lactobacilli and strongly stimulate their biofilm formation (218-142%) and the tensioactive activity (117-104%). Our results are promising for design of selective antimicrobial containing protective effects for beneficial lactobacilli known as health promoters.