Deleterious Metabolic Effects of High Fructose Intake: The Preventive Effect of Lactobacillus kefiri Administration

ZUBIRÍA, MARÍA; GAMBARO, SABRINA; REY, MARÍA; CARASI, PAULA; SERRADELL, MARÍA; GIOVAMBATTISTA, ANDRÉS

Nutrients

Nutrients Editorial Office

Lugar: Basel; Año: 2017 vol. 9

Modern lifestyle and diets have been associated with metabolic disorders and an imbalancein the normal gut microbiota. Probiotics are widely known for their health beneficial propertiestargeting the gut microbial ecosystem. The aim of our study was to evaluate the preventive effectof Lactobacillus kefiri (L. kefiri) administration in a fructose-rich diet (FRD) mice model. Mice wereprovided with tap water or fructose-added (20% w/v) drinking water supplemented or not withL. kefiri. Results showed that probiotic administration prevented weight gain and epidydimal adiposetissue (EAT) expansion, with partial reversion of the adipocyte hypertrophy developed by FRD.Moreover, the probiotic prevented the increase of plasma triglycerides and leptin, together with theliver triglyceride content. Leptin adipocyte secretion was also improved by L. kefiri, being able torespond to an insulin stimulus. Glucose intolerance was partially prevented by L. kefiri treatment(GTT) and local inflammation (TNFα; IL1β; IL6 and INFγ) was completely inhibited in EAT. L. kefirisupplementation generated an impact on gut microbiota composition, changing Bacteroidetes andFirmicutes profiles. Overall, our results indicate that the administration of probiotics prevents thedeleterious effects of FRD intake and should therefore be promoted to improve metabolic disorders.