Human papillomavirus detection in Corrientes,Argentina: High prevalence of genotype 58 and its phylodynamics

MARIN, H; TORRES, C.; DELUCA, G; MBAYED, V.A.

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2015 vol. 47 p. 302 - 311

0325-7541

Human papillomavirus (HPV) has the highest mortality rate due to cervical cancerin Northeastern Argentina. The aim of this work was to detect and characterize HPV in samplesfrom the Province of Corrientes, Argentina. HPV detection and typing was performed usingPCR-RFLP on samples with different cervical lesions (n = 255). Seventeen viruses typified as HPV-58 were sequenced (E6 and E7 genes) and mutations were analyzed. HPV DNA was detectedin 56.1 % of the cervical lesions (143/255). Twenty-two different HPV types were detected.The type most frequently found among the total number of samples and HPV-positive sampleswas HPV-16 (14.5 % and 25.9 %, respectively), followed by HPV-58 (8.2 %/14.7 %, respectively),which is also considered a high-risk viral type. Increased severity of the cytological status wasassociated with greater rates of HPV detection and, especially, with the detection of greaterrates of high-risk types. In addition, the evolutionary dynamics of the alpha-9 species group andHPV-58 was studied. All HPV-58 viruses reported in this work belonged to lineage A, sublineageA2. The phylodynamic analysis indicated that diversification of main groups within lineage Amight have accompanied or preceded human migrations across the globe. Given that the mostprevalent viruses found belonged to high-risk HPV types, some concerns might arise about theextent of cross protection of the vaccines against the types not included in their desig