Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia

MOLINA, N.B., POLVERINO, D., MINVIELLE, M.C., APEZTEGUÍA, M., AGUILAR O.M., BASUALDO J.A,

PARASITOLOGíA LATINOAMERICANA

Sociedad Chilena de Parasitología

Lugar: Santiago de Chile; Año: 2006 vol. 61 p. 133 - 137

0717-7704

The efficiency of lysis procedures and DNA purification treatments of Giardia lamblia trophozoites were evaluated regarding the breaking-up efficiency, quantity and pureness of DNA, processing time and costs. Five lysis methods (distilled water and heating; distilled water, heating and proteinase K; buffer D Lysis; buffer E lysis, and commercial kit), and three DNA purification methods (phenol:chloroform:isoamilic, Chelex 100 and commercial kit) were tested. The obtained data were statistically analyzed. The buffer E lysis and Chelex combination was simple and economic method which produced high DNA performance with low pureness. Commercial kit was simple and expensive method which generated low yields of DNA with appropriate purity level for molecular analysisThe efficiency of lysis procedures and DNA purification treatments of Giardia lamblia trophozoites were evaluated regarding the breaking-up efficiency, quantity and pureness of DNA, processing time and costs. Five lysis methods (distilled water and heating; distilled water, heating and proteinase K; buffer D Lysis; buffer E lysis, and commercial kit), and three DNA purification methods (phenol:chloroform:isoamilic, Chelex 100 and commercial kit) were tested. The obtained data were statistically analyzed. The buffer E lysis and Chelex combination was simple and economic method which produced high DNA performance with low pureness. Commercial kit was simple and expensive method which generated low yields of DNA with appropriate purity level for molecular analysis