INVESTIGADORES
AGUILAR Orlando Mario
artículos
Título:
Rhizobium tropici GuaB activity is required during the early stages of nodulation of host plants with determinate nodules but dispensable for the Sinorhizobium meliloti-alfalfa symbiotic interaction
Autor/es:
COLLAVINO, M.M., RICCILLO, P.M., GRASSO, D.H., CRESPI, M., AND AGUILAR, O.M
Revista:
MOLECULAR PLANT-MICROBE INTERACTIONS
Editorial:
The International Society for Molecular Plant-Microbe Interactions
Referencias:
Lugar: The American Phytopathological Society; Año: 2005 vol. 18 p. 742 - 750
ISSN:
0894-0282
Resumen:
The guaB mutant strain Rhizobium tropici CIAT8999-10T
is defective in symbiosis with common bean, forming nodules
that lack rhizobial content. In order to investigate the
timing of the guaB requirement during the nodule formation
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
is defective in symbiosis with common bean, forming nodules
that lack rhizobial content. In order to investigate the
timing of the guaB requirement during the nodule formation
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
guaB mutant strain Rhizobium tropici CIAT8999-10T
is defective in symbiosis with common bean, forming nodules
that lack rhizobial content. In order to investigate the
timing of the guaB requirement during the nodule formation
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
guaB requirement during the nodule formation
on the host common bean by the strain CIAT899-10.T,
we constructed gene fusions in which the guaB gene is expressed
under the control of the symbiotic promoters
under the control of the symbiotic promoters
guaB gene is expressed
under the control of the symbiotic promoters
nodA, bacA, and nifH. Our data indicated that the guaB is
required from the early stages of nodulation because full
recovery of the wild-type phenotype was accomplished by
the nodA-guaB fusion. In addition, we have constructed a
required from the early stages of nodulation because full
recovery of the wild-type phenotype was accomplished by
the nodA-guaB fusion. In addition, we have constructed a
A, bacA, and nifH. Our data indicated that the guaB is
required from the early stages of nodulation because full
recovery of the wild-type phenotype was accomplished by
the nodA-guaB fusion. In addition, we have constructed anodA-guaB fusion. In addition, we have constructed a
guaB mutant derived from Sinorhizobium meliloti 1021,
and shown that, unlike R. tropici, the guaB S. meliloti
and shown that, unlike R. tropici, the guaB S. meliloti
B mutant derived from Sinorhizobium meliloti 1021,
and shown that, unlike R. tropici, the guaB S. melilotiR. tropici, the guaB S. meliloti
mutant is auxotrophic for guanine and induces wild-type
nodules on alfalfa and Medicago truncatula. The guaB R.
tropici mutant also is defective in its symbiosis with Macroptilium
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
tropici mutant also is defective in its symbiosis with Macroptilium
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
Medicago truncatula. The guaB R.
tropici mutant also is defective in its symbiosis with Macroptilium
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
mutant also is defective in its symbiosis with Macroptilium
atropurpureum and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
and Vigna unguiculata but normal
with Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
Leucaena leucocephala. These results show that the
requirement of the rhizobial guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.
be associated with host plants that form determinate type
of nodules.
guaB for symbiosis is found to
be associated with host plants that form determinate type
of nodules.