High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

RAVASI, PABLO; BRAIA, MAURICIO; EBERHARDT, FLORENCIA; ELENA, CLAUDIA; CERMINATI, SEBASTIÁN; PEIRÚ, SALVADOR; CASTELLI, MARÍA EUGENIA; MENZELLA, HUGO

JOURNAL OF BIOTECHNOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 216 p. 142 - 148

0168-1656

Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5 g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme.