Molecular confirmation of the relationship between Candida guilliermondii Fks1p naturally occurring amino acid substitutions and its intrinsic reduced echinocandin susceptibility

DUDIUK, CATIANA; MACEDO, DAIANA; LEONARDELLI, FLORENCIA; THEILL, LAURA; CABEZA, MATIAS; GAMARRA, SOLEDAD; GARCIA, GUILLERMO

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2017

0066-4804

Candida guilliermondii shows intrinsic reduced echinocandin susceptibility.It harbors two polymorphisms (L633M and T634A) in the Fks1p hot spot 1 region.Our objective was to confirm that the reduced echinocandin susceptibility ofC. guilliermondii is due to those naturally occurring substitutions. We constructed a Saccharomyces cerevisiae mutant in which a region of the FKS1 gene (including hot spot 1) was replaced with that from C. guilliermondii. The chimeric mutants showed 32-fold increases in echinocandin MIC values, confirming the hypothesis.