PERSONAL DE APOYO
ROLNY Ivanna Sabrina
artículos
Título:
Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
Autor/es:
HUGO, A.A.; ROLNY I.S.; ROMANIN, D.; PEREZ P.F.
Revista:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017
ISSN:
0959-3993
Resumen:
Abstract Citrobacter rodentium is a specific murineenteropathogen which causes diarrheal disease characterizedby colonic hyperplasia and intestinal inflammation.Recruitment of neutrophils and macrophages constitute akey step to control the infection. Since modulation of theactivity of professional phagocytic cells could contribute toimprove host´s defences against C. rodentium, we investigatedthe effect of Lactobacillus delbrueckii subsp. lactis(strain CIDCA 133) on the interaction between murinemacrophages (RAW 264.7) and C. rodentium. Phagocytosis,surface molecules and inducible nitric oxide synthase(iNOs) expression were determined by flow cytometry.Reactive oxygen species (ROS) were assessed by fluorescencemicroscopy. The presence of lactobacilli increased phagocytosis of C. rodentium whereas C. rodentium hadno effect on lactobacilli internalization. Survival of internalizedC. rodentium diminished when strain CIDCA 133was present. CD-86, MHCII, iNOs expression and nitriteproduction were increased when C. rodentium and lactobacilliwere present even though strain CIDCA 133 alonehad no effect. Strain CIDCA 133 led to a strong inductionof ROS activity which was not modified by C. rodentium.Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133)is able to increase the activation of murine macrophagesinfected with C. rodentium. The sole presence of lactobacilliis enough to modify some stimulation markers (e.g.ROS induction) whereas other markers require the presenceof both bacteria; thus, indicating a synergistic effect.