Analyzing the Hydrodynamic and Crowding Evolution of Aqueous Hydroxyapatite-Gelatin Networks: Digging Deeper into Bone Scaffold Design Variables

SARTUQUI JAVIER; D'ELÍA NOELIA LAURA; GRAVINA ANABELA NOEL; MESSINA PAULA VERÓNICA

BIOPOLYMERS

JOHN WILEY & SONS INC

Lugar: New York; Año: 2015 vol. 103 p. 393 - 405

0006-3525

The hydration of the polypeptide network is a determi-nant factor to be controlled on behalf of the design of pre-cise functional tissue scaffolding. Here we present an exhaustive study of the hydrodynamic and crowding evo-lution of aqueous gelatin-hydroxyapatite systems with the aim of increasing the knowledge about the biomimesis of collagen mineralization; and how it can be manipulated for the preparation of collagenous derived frameworks with specific morphological characteristics. The solution?s density and viscosity evaluation measurements in combi-nation with spectroscopic techniques revealed that there is a progressive association of protein chain that can be influenced by the amount of hydroxyapatite nanorods. Gelatin and additives? concentration effect on the mor-phology of the gelatin scaffolds was investigated. Trans-verse and longitudinal sections of the obtained scaffolds were taken and analyzed using optical microscopy. It can be seen that the porous size and shape of gelatin assem-blies can be easily adjusted by controlling the gelatin/HAp ratio in the solution used as template in agreement with our statement.