Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment

FELICE, JUAN IGNACIO; SCHURMAN, LEÓN; MCCARTHY, ANTONIO DESMOND; SEDLINSKY, CLAUDIA; AGUIRRE, JOSÉ IGNACIO; CORTIZO, ANA MARÍA

DIABETES RESEARCH AND CLINICAL PRACTICE

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2017 vol. 126 p. 202 - 213

0168-8227

AimsDeleterious effects ofmetabolic syndrome (MS) on bone are still controversial. In this study weevaluated the effects of a fructose-induced MS, and/or an oral treatment withmetformin on the osteogenic potential of bone marrow mesenchymal stromal cells(MSC), as well as on bone formation and architecture.Methods32 male 8 week-old Wistar rats were assigned to four groups: control(C), control plus oral metformin (CM), rats receiving 10% fructose in drinkingwater (FRD), and FRD plus metformin (FRDM). Samples were collected to measureblood parameters, and to perform pQCT analysis and static and dynamichistomorphometry. MSC were isolated to determine their osteogenic potential.ResultsMetformin improved blood parameters in FRDM rats. pQCT and static anddynamic histomorphometry showed no significant differences in trabecular and corticalbone parameters among groups. FRD reduced TRAP expression and osteocyte densityin trabecular bone and metformin only normalized osteocyte density. FRDdecreased the osteogenic potential of MSC and metformin administration couldrevert some of these parameters.ConclusionsFRD-induced MS shows reduction in MSC osteogenic potential, in osteocytedensity and in TRAP activity. Oral metformin treatment was able to preventtrabecular osteocyte loss and the reduction in extracellular mineralizationinduced by FRD-induced MS.