Lactobacillus rhamnosus RC007 has immune-stimulatory properties and ameliorating effects on TNBS induced colitis in mice

DOGI, C.A; GARCÍA, G; DE MORENO DE LEBLANC A; GRECO C.; CAVAGLIERI, LILIA

Beneficial Microbes

Wagenigen Academic

Año: 2016 vol. 7 p. 539 - 547

1876-2883

Aims: To evaluate in vivo the beneficial properties of Lb. rhamnosus RC007 when administered to healthy and trinitro benzene sulfonic acid (TNBS) colitis induced BALB/c mice. Methods and Results: The administration of Lb. rhamnosus RC007 to healthy mice during 10 days increased the phagocytic activity of peritoneal macrophages and the number of IgA+ cells in the lamina propria of the small intestine. Significant increases of MCP-1; IL-10 and TNFconcentrations, and in the ratio between anti- and pro-inflammatory cytokines (IL-10/TNFα) were observed in intestinal fluids after bacteria administration. In the inflammation model, less body weight loss, macroscopic and histological damages in the large intestine were accompanied byincreased IL-10/TNFα ratio in the intestinal fluids of mice from L. rhamnosus-TNBS group when compared to TNBS group. Conclusions: In healthy host, the oral administration of Lb. rhamnosus RC007 kept the gut immune system stimulated allowing a faster response to noxious stimulus. Mice that received L. rhamnosus RC007 also decreased the severity of the intestinal inflammation. Significance and impact of the study: Lb. rhamnosus RC007 is a potential probiotic bacterium that can exert beneficial effect as supplement for animal feed, by improving the immune status in healthy host, and by providing therapeutic benefits to infected/inflamed animals.