FORMULATION AND QUALITY CONTROL OF SEMIS0LID CONTAINING HARMLESS BACTERIA BY-PRODUCTS: CHRONIC WOUNDS PRO-HEALING ACTIVITY.

MARIA EUGENIA SESTO CABRAL; ALBERTO NICOLÁS RAMOS VERNIERI; ALEXANDRE JOSE MACEDO; DANIELLE TRENTIN; YANINNE TRETER; MANZO, RH; VALDEZ, JUAN CARLOS

PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2015 vol. 20 p. 911 - 918

1083-7450

Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process, being infection one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. LAPSs Chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This paper shows a pharmaceutical dosage form designed by using LAPS as an active pharmaceutical ingredient, with pro-healing activity and its quality control. Pharmacotechnical assays and several antimicrobial assays were asdapted to demonstrate that the vehicle used does not modify the LAPS activities. The formulations using 100% of LAPS (F100), showed a smooth and homogeneous appearance, were easily spreadable and demonstrate acceptable bioadhesion and fair mechanical properties. From the in vitro release assays was found an adequate release from the carrier matrix. Furthermore, F100 is stable and maintains its antipathogenic activity for a period of 6 months. We propose for the treatment of chronic wounds F100 application. This new treatment provides P. aeruginosa antipathogenic activity as a combination of biocidal and anti-virulence actions.