In vitro protein synthesis is affected by the herbicide 2,4-dichlorophenoxyacetic acid in Azospirillum brasilense

RIVAROLA,V.; FABRA, ADRIANA; MORI,G.; BALEGNO,H.

TOXICOLOGY

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 1992 vol. 73 p. 71 - 79

0300-483X

The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on growth and protein, DNA and RNA synthesisof Azospirillum brasilense Cd were studied. At a concentration of 1 mM, 2,4-D inhibited cell growth, aneffect that was reversed either by transferring bacteria to a control (2,4-D-free) medium or to a 2,4-Dtreatedmedium supplemented with polyamines. The herbicide also affected in vitro protein synthesis,either when Azospirillum brasilense Cd´s own cellular mRNA or an artificial mRNA was used. This effectwas also reversed by the addition of polyamines to the 2,4-D-treated medium. Similar results were observedwhen DNA synthesis was studied in synchronous cultures. Taking into account the effects of this herbicideon animal cells (V.A. Rivarola and H.F. Balegno, Toxicology, 68 (1991) 109) we postulate that themechanism of action of 2,4-D