Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp. S119

LUDUEÑA, L; ANZUAY, M.; ANGELINI, J.; BARROS,G.; LUNA,M.; MONGE,M.; FABRA, A.; TAURIAN, T.

SYMBIOSIS

SPRINGER

Lugar: Berlin; Año: 2016

0334-5114

The aim of this study was to analyze if cofactorpyrroquinoline quinone from Serratia sp. S119 is involved inthe inorganic phosphate solubilization mechanism and in itsability to promote the plant growth. Site directed mutagenesiswas performed to obtain a pqqE- minus mutant of strainSerratia sp. S119. The phosphate solubilization ability, gluconateand PQQ production of the mutant Serratia sp. RSL(pqqE-) was analyzed.Mutant RSL (pqqE-) showed significantdecrease in P soluble and gluconic acid levels produced andundetectable levels of PQQ cofactor compared with wild-typestrain. Complementation with synthetic PQQ cofactor restoredP solubilization and gluconate production reaching the levelsproduced by wild-type strain. PqqE gene sequence indicatedthat it is highly conserved within Serratia strains and its productshows conserved motifs found in other PqqE proteins ofseveral bacteria. The effect of the inoculation of the PQQmutanton peanut and maize plants was evaluated in pot assays.Plants growth parameters showed no differences among thedifferent treatments indicating that PQQ from Serratia sp.S119 is not involved in the growth promotion of these plants.PQQ cofactor is essential for phosphate solubilization ability of Serratia sp. S119 but is not required for growth promotion ofpeanut and maize plants