INVESTIGADORES
ETCHENIQUE Roberto Argentino
artículos
Título:
"RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines
Autor/es:
FINO ELODIE; ARAYA ROBERTO; PETERKA DARCY; SALIERNO MARCELO; ETCHENIQUE ROBERTO; YUSTE RAFAEL
Revista:
Frontiers in Neural Circuits
Editorial:
Frontiers Research Foundation
Referencias:
Año: 2009 vol. 3 p. 2 - 3
ISSN:
1662-4548
Resumen:
We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound
based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths
and releases glutamate after one- or two-photon excitation. It has high quantum effi ciency and
can be used at low concentrations, partly avoiding the blockade of GABAergic transmission
present with other caged compounds. Two-photon uncaging of RuBi-Glutamate has a high spatial
resolution and generates excitatory responses in individual dendritic spines with physiological
kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used
to depolarize and fi re pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore
enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light
sources, achieving single cell, or even single spine, precision.