Construction and in vitro testing of a cellulose dura mater graft

SEBASTIÁN KORNFELD; PABLO M. AJLER; CASTRO GUILLERMO; EZEQUIEL GOLDSCHMIDT; MARINA VALINOTI; CLAUDIO YAMPOLSKY; CACICEDO MAXIMILIANO L.; PABLO ARGIBAY; MARCELO IELPI; JORGE RASMUSSEN

NEUROLOGICAL RESEARCH.

MANEY PUBLISHING

Lugar: London; Año: 2016 vol. 38 p. 25 - 31

0161-6412

Introduction: Cerebrospinal fluid (CSF) leaks are a common complication after cranial and spinal surgery and are associated with increased morbidity. Despite continuous research in this field, this problem is far from solved. In this paper, we describe the construction and testing of a bacterial cellulose (BC) membrane as a new dural patch.Materials and Methods: The synthesis of BC was performed using Gluconacetobacter hansenii (ATCC 23769) and films were sterilized by autoclaving. The membranes were seeded with human dural fibroblasts. Growth, shape, and cell viability were assessed after 4 weeks.Results: Normally shaped fibroblasts were seen on the BC grafts; confocal microscopy showed cells inside the structure of the mesh. Both viable and nonviable cells were present. Cellular attachment and viability were confirmed by replating of the membranes.Discussion: BC membranes are used in clinical practice to improve skin healing. In the presence of water, they form an elastic, nontoxic, and resistant biogel that can accommodate collagen and growth factors within their structure, thus BC is a good candidate for dural graft construction.