INVESTIGADORES
TEKIEL Valeria Sonia
artículos
Título:
The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs.
Autor/es:
FLORIDIA YAPUR, N; MONJE RUMI, M; RAGONE, P; LAUTHIER, J; TOMASINI, N; ALBERTI D AMATO, A; DIOSQUE, P; CIMINO, R; MARCO, D; BARROSO, P; SÁNCHEZ, DO; NASSER, J; TEKIEL V
Revista:
PARASITOLOGY
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Lugar: Cambridge; Año: 2016 vol. 143 p. 1382 - 1389
ISSN:
0031-1820
Resumen:
In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect ELISA (Enzyme Linked Immunosorbent Assay) assays were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations where infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.