INVESTIGADORES
FAILLACE Maria paula
artículos
Título:
Daily variations in 2-125I-melatonin specific binding in the golden hamster retina.
Autor/es:
11. FAILLACE MP., DE LAS HERAS MA., KELLER SARMIENTO MI., ROSENSTEIN RE.
Revista:
NEUROREPORT
Editorial:
Lippincot
Referencias:
Año: 1995 vol. 7 p. 141 - 144
ISSN:
0959-4965
Resumen:
Daily variations in melatonin content and 2-[125I]melatonin specific
binding in retinas of golden hamsters were studied. Both parameters
showed significant variations throughout the 24 h period. Maximal
specific binding was observed at 24.00 h, while melatonin content
peaked at 04.00 h. Saturation studies performed at 12.00 and 24.00 h
indicated that the maximal concentration of 2-[125I]melatonin binding
sites (Bmax) was significantly higher at 24.00 h than at 12.00 h,
whereas the dissociation constant (Kd) remained unchanged. As
2-[125I]melatonin specific binding decreased at times when retinal
melatonin content was high, these findings suggest that daily
variations in retinal melatonin levels may be implicated in the
regulation of the density of melatonin binding sites, probably through
mechanisms of up- and down-regulation induced by melatonin on its own
binding sites.