BACTERICIDAL SECONDARY METABOLITES PRODUCE BY Fusarium sp. AGAINST Staphylococcus aureus

CAMERANESI M; MARCINKEVICIUS K; LUCIARDI C; SORIA NAJ; ARENA ME; BORGES I

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 1 - 1

0327-9545

Entomopathogenic fungus (EF) causes the death of the host insect. EF are a rich source of natural bioactive compounds. When the insect dies several microorganisms that grow normally in the exoskeleton surface, as well as, in nearly all the epithelium, such as Staphylococcus aureus try to degrade the insect body. However, the EF consumes the insect. The objective was determined if the EF Fusarium sp. 3300 (NRRL 25102) inhibit the growth and biofilm formation of S. aureus ATCC 6538. The fungus was cultured on a dextrose potato broth in the absence (A) and in the presence of 2% (w/v) pups of Ceratitis capitata (B), using as a control the medium with insect (C). After 15 days incubation, the supernatants was separated of the mycelium by filtration, the supernatant was extracted with ethyl acetate and the FM produce in the different media were analyzed by TLC. Eighteen eluates were obtained in the B condition; eight of them were produced by the insect induction. The extract obtained from A and B conditions, as well as, four elautes produced by induction have bactericidal activity against S. aureus ATCC 6538. The production of substances in presence of insect-derived material suggested that new antimicrobial compounds are produce when the fungus infects the insect. This substances of eukaryotic origin, and normally present into the environmental, should be study as potential antibiotics