METABOLITES WITH ANTIBIOFILM PROPERTY FORMED BY Aspergillus parasiticus

LUCIARDI C; CARTAGENA E; BARDÓN A; ARENA ME

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 1 - 1

0327-9545

modify the concentrations of fungal metabolite (FM) produce by entomopathogenic fungus. The objective was determined the antibacterial and antibiofilm activity of FM produced by Aspergillus parasiticus MEA1 on Pseudomonas aeruginosa ATCC 27853. The fungus was cultured on a dextrose potato broth in the absence (A) and in the presence of 2% (w/v) cuticle of Spodoptera frugiperda (B), using as a control the medium with insect (C). After 15 days incubation, the supernatants was separated of the mycelium by filtration, both were extracted with chloroform and the FM produce in the different media were analyzed by TLC and GC-MS. The extracts chloroform (EC) obtained from B condition were separated by TLC, and the antibacterial and antibiofilm activity determined. In the EC of the mycelium B the main metabolite found was 2-oleilglicerol (28.67%). On the other hand, the EC of liquid medium had a higher concentration of undecyl-4-fluorobenzoate. The EC mycelium B inhibited 41% the biofilm formation of P. aeruginosa at 100 µg/mL and the three elautes obtained by TLC preparative: less-polar (contained 12% of 17-pentatriaconteno), intermediate-polarity (comprising 35% of 2-(4-bromophenyl) -2-oxoethylbenzoate), and polar-eluate (60% ergosterol) inhibiting 43, 20 and 30% the biofilm formation. The presence of insects induced the formation of FM able to inhibit the biofilm of human pathogen P. aeruginosa; these FM should be evaluated as potential antipathogenics.