Ex vivo treatment with a polyphenol-enriched cocoa extract ameliorates myocardial infarct and postischemic mitochondrial injury in normotensive and hypertensive rats

GONZÁLEZ ARBELÁEZ LF; CIOCCCI PARDO A; CIOCCI PARDO, A; FANTINELLI JC; CALDIZ, CI; RIOS J; SCHINELLA GR; MOSCA SM

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2016

0021-8561

Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardialpostischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR).Isolated hearts were submitted to 110 min of perfusion or 20-min stabilization, 30-min global ischemiaand 60-min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, thereduced glutathione (GSH) and the expression of phospho-Akt, P-GSK-3β and P-eNOS were assessed. Inisolated mitochondria the Ca2+38 - mediated response of mitochondrial permeability transition pore (mPTP),membrane potential (ψm) and superoxide production were determined. PCE decreased infarct size,partly preserved GSH, increased the P-Akt, P-GSK-3β and P-eNOS contents, improved mPTP responseto Ca2+ , decreased the superoxide production and restored ψm.These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest thatAkt/GSK-3β/eNOS dependent pathways are involved.