Genetic variability and recombination analysis of the coat protein gene of Strawberry mild yellow edge virus

ADA KARINA TORRICO; MARCOS GIOVANI CELLI; EVA ENCARNACION CAFRUNE; DANIEL SANTIAGO KIRSCHBAUM; VILMA CECILIA CONCI

AUSTRALASIAN PLANT PATHOLOGY

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2016

0815-3191

Strawberry mild yellow edge virus (SMYEV) has been detected in most of the strawberry production regions worldwide. However, little is known about of differences between distinct isolates. The aim of this study was enhance the knowledge about the genetic variability of different SMYEV isolates, exploring the phylogenetic relationships and assessing recombinant events among them. The coat protein (CP) gene of 12 Argentine SMYEV isolates was sequenced. It was 729 nucleotides (nt) in all of the isolates, encoding a protein of 242 amino acids (aa). Argentine isolates shared 81.5 - 99.6 % nucleotide identity. The comparison of these isolates with 30 SMYEV sequences published in GenBank from other countries revealed an identity ranging between 81.6 and 99 %. The phylogenetic analysis showed the presence of possible four subgroups, with the Argentine isolates being included in all of them. Recombination analysis indicated that 16-2 Argentina and AJ577342 Chile isolates are recombinant and that they were originated through recombination events in which parts of the genome were exchanged between different SMYEV sequences.