Novel Augmented Parallel Factor Model for Four-Way Calibration of High-Performance Liquid Chromatography-Fluorescence Excitation-Emission Data

ARSENIO MUÑOZ DE LA PEÑA; VALERIA LOZANO; SANTIAGO BORTOLATO; ALEJANDRO OLIVIERI

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015

0169-7439

A new augmented parallel factor analysis model (Augmented PARAFAC) is presented, inspired in the useful augmentation concept employed in multivariate curve resolution- alternating least-squares (MCR-ALS), applicable to calibration based in non-quadrilinear four- way data, such as those produced by the high-performance liquid chromatography with matrix excitation-emission fluorescence detection. The new model involves creating an augmented three-way array in the elution time direction, containing data for the calibration sample set and for each of the test samples, subsequently analyzed with an Augmented PARAFAC version. To test the properties of this approach, chromatographic data were simulated with several different degrees of overlapping and misalignment among the chromatographic peaks. Additionally, experimental data from olive oil samples were tested with the new model, aimed at the quantitation of the level of chlorophylls and pheophytins. The results were compared with those obtained by data processing with MCR-ALS. Relative prediction errors (%) were: Augmented PARAFAC, 9.7, 21.0, 14.7 and 9.3, MCR-ALS, 5.9, 14.5, 20.0 and 14.7 for Chl a, Chl b, Phe a and Phe b, respectively, for concentrations in the range 0.00-1.00 µg mL ?1 . Both MCR-ALS and Augmented PARAFAC allow one to obtain a detailed and realistic description of the analyzed samples, in terms of pure elution time, excitation and emission spectral profiles, which can be independently retrieved for every component.