INVESTIGADORES
BARBEITO Claudio Gustavo
artículos
Título:
Changes in carbohydrate expression in the cervical spinal cord of rats during aging
Autor/es:
LOZZA F; CHINCHILLA L; BARBEITO CG; GOYA RG; GIMENO EJ; PORTIANSKY EL
Revista:
Neuropathology
Editorial:
Japanese Society of Neuropathology
Referencias:
Lugar: Tokyo; Año: 2009 vol. 29 p. 258 - 262
Resumen:
Aging is a process where histochemical changes occur.Some
of these may consist of age-dependent loss of expression of
some cell markers.Conversely, cell markers not expressed in
young animals may be detectable in their older counterparts.
Histochemical age changes in carbohydrate profiles in
the spinal cord have not been documented. In order to fill
this information gap lectin histochemistry and image analysis
were used to characterize the histochemical age changes
occurring in the cervical segments of the rat spinal cord.
From a battery of 11 lectins, the more important age changes
were detected with Glicine maximus (SBA)-lectin. Thus,
SBA-lectin neuronal staining which was moderately positive
in the cervical segments of young animals was negative
in old rats. In contrast the same lectin which did not react
with the ependyma of young animals strongly bound to the
ependyma of senescent rats. None of the tested lectins
bound to glial cells, either in young or old animals. In no case
the senile animals evidenced anatomopathological changes.
We conclude that although in the aged spinal cord changes
in lectin histochemical binding patterns occur, they do not
reflect a pathologic situation.
SBA-lectin neuronal staining which was moderately positive
in the cervical segments of young animals was negative
in old rats. In contrast the same lectin which did not react
with the ependyma of young animals strongly bound to the
ependyma of senescent rats. None of the tested lectins
bound to glial cells, either in young or old animals. In no case
the senile animals evidenced anatomopathological changes.
We conclude that although in the aged spinal cord changes
in lectin histochemical binding patterns occur, they do not
reflect a pathologic situation.
SBA-lectin neuronal staining which was moderately positive
in the cervical segments of young animals was negative
in old rats. In contrast the same lectin which did not react
with the ependyma of young animals strongly bound to the
ependyma of senescent rats. None of the tested lectins
bound to glial cells, either in young or old animals. In no case
the senile animals evidenced anatomopathological changes.
We conclude that although in the aged spinal cord changes
in lectin histochemical binding patterns occur, they do not
reflect a pathologic situation.
Glicine maximus (SBA)-lectin. Thus,
SBA-lectin neuronal staining which was moderately positive
in the cervical segments of young animals was negative
in old rats. In contrast the same lectin which did not react
with the ependyma of young animals strongly bound to the
ependyma of senescent rats. None of the tested lectins
bound to glial cells, either in young or old animals. In no case
the senile animals evidenced anatomopathological changes.
We conclude that although in the aged spinal cord changes
in lectin histochemical binding patterns occur, they do not
reflect a pathologic situation.