INVESTIGADORES
GONZALEZ DENISELLE Maria Claudia
artículos
Título:
Expression and cellular localization of the classical progesterone receptor in healthy and amyotrophic lateral sclerosis affected spinal cord.
Autor/es:
GARGIULO MONACHELLI G; CAMPOS-MELO D; DROPPELMANN C; KELLER B; LEYSTRA LANTZ; DE NICOLA A. F.; GONZALEZ DENISELLE MC; VOLKENNING K; STRONG MJ
Revista:
EUROPEAN JOURNAL OF NEUROLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 21 p. 273 - 280
ISSN:
1351-5101
Resumen:
Background and purpose: Previous studies have suggested that elevated progesteronelevels are associated with a slower disease course in ALS. Given that the effects ofprogesterone are mediated in part by the classical progesterone receptor (PR), we haveexamined the expression and cellular localization of the A and B isoforms (PR-A and PRB,respectively) of the PR in control (neuropathologically normal) and ALS-affectedspinal cord (SC).Methods: We performed semi-quantitative RT-PCR, immunohistochemistry (IHC) andimmunofluorescence (IF) in the cervical and lumbar SC of post-mortem ALS patients(n=19) and control subjects (n=10). Primers and antibodies used allowed the detection ofboth PR-A and PR-B isoforms together (PR-A+B) or PR-B isoform alone.Results: Lumbar PR-A+B and cervical PR-B mRNA expression were significantlyhigher in ALS than controls. PR-A was inferred to be expressed at higher levels in thelumbar and at lower levels in the cervical SC of ALS patients compared with controls. Inboth ALS and controls, PR-A+B immunoreactivity (IR) was occasionally detected inmotor neurons. In contrast, PR-A+B IR was prominent in axonal processes and vessels.This was more evident in nerve roots and large arteries in ALS compared with controls.We also observed colocalization of PR-A+B with markers of neurons, axonal processesand vascular endothelium.Conclusions: We provide evidence that both PR-A and PR-B isoforms are expressed inthe human SC, with some regional variation in isoform expression between ALS andcontrols. The IR was more prominent in nerve roots and large arteries in ALS, suggestinga potential role in the degenerative process.
