Increased astrocyte reactivity in the hippocampus of murine models of type I diabetes: the non-obese diabetic (NOD) and the streptozotocin-treated mice.

SARAVIA FE; REVSIN Y; GONZALEZ DENISELLE MC; GONZALEZ SL; ROIG P; LIMA A; HOMO-DELARCHE F; DE NICOLA A. F.

BRAIN RESEARCH

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2002 vol. 957 p. 345 - 353

0006-8993

Diabetes can be associated with cerebral dysfunction in humans and animal models of the disease. Moreover, brain anomalies and alterations of the neuroendocrine system are present in type 1 diabetes (T1D) animals, such as the spontaneous nonobese diabetic (NOD)mouse model and/or the pharmacological streptozotocin (STZ)-induced model. Because of the prevalent role of astrocytes in cerebral glucose metabolism and their intimate connection with neurones, we investigated hippocampal astrocyte alterations in prediabetic anddiabetic NOD mice and STZ-treated diabetic mice. The number and cell area related to the glial fibrillary acidic protein (GFAP)-immunoreactive astrocytes were quantified in the stratum radiatum region of the hippocampus by computerized image analysis inprediabetic (2, 4 and 8 weeks of age) and diabetic (16-week-old) NOD female mice, age and sex-matched lymphocyte-deficient NODscid and C57BL/6 control mice and, finally, STZ-induced diabetic and vehicle-treated nondiabetic 16-week-old C57BL/6 female mice.Astrocyte number was higher early in life in prediabetic NOD and NODscid mice than in controls, when transient hyperinsulinemia and 1 low glycemia were found in these strains. The number and cell area of GFAP cells further increased after the onset of diabetes in NOD1 mice. Similarly, in STZ-treated diabetic mice, the number of GFAP cells and cell area were higher than in vehicle-treated mice. In conclusion, astrocyte changes present in genetic and pharmacological models of T1D appear to reflect an adaptive process to alterations ofglucose homeostasis.