Involvement of PAPP-A and IGFR1 in Cystic Ovarian Disease in Cattle

RODRIGUEZ, MF; COLOMBERO, M; AMWEG A; HUBER E; GAREIS N; SALVETTI NR; ORTEGA HH; REY, F

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 50 p. 659 - 668

0936-6768

Cystic ovarian disease (COD) is one of the main causes ofinfertility in dairy cattle. It has been shown that intra-ovarianfactors, such as members of the insulin-like growth factor(IGF) system, may contribute to follicular persistence. Thebioavailability of IGF to initiate its response by binding tospecific receptors (IGFRs) depends on interactions withrelated compounds, such as pregnancy-associated plasmaprotein A (PAPP-A). The aim of this study was to determineIGFR1 and PAPP-A expression both in follicles at differentstages of development and in cysts, to evaluate the roles in theetiopathogenesis of COD in cattle. The mRNA expression ofPAPP-A was higher in granulosa cells of large tertiary folliclesthan in cysts, whereas the protein PAPP-A present in thefollicular fluid from these follicles showed no differences.Although no PAPP-A mRNA expression was detected insmaller tertiary follicles, in their follicular fluid, this proteasewas detected in lesser concentration than in cysts. The mRNAexpression of IGFR1 was lower in granulosa cells from cysticfollicles than in those from tertiary ones. However, the proteinexpression of this receptor presented the highest levels in cysticstructures, probably to increase the possibility of IGFresponse. The data obtained would indicate that animals withCOD have an altered regulation of the IGF system in theovary, which could be involved in the pathogenesis of thisdisease in cattle.