Arbuscular mycorrhizal fungal diversity in rhizosphere spores versus roots of an endangered endemic tree from Argentina: Is fungal diversity similar among forest disturbance types?

SOTERAS, F; COUTINHO MOREIRA, B; GRILLO, G; PASTOR, N; MENDES CARNEIRO, F; RUELA DE CARVALHO, D; RENISON, D; MEGUMI KASUYA, MC; DE SOUZA, F; BECERRA, AG

APPLIED SOIL ECOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 98 p. 272 - 277

0929-1393

In this study wehypothesized that the AMF community of the rhizosphere spores will be resilientagainst anthropogenic disturbances and that AMF associated with roots willbelong to the same functional group of its host. To test that, we compare thearbuscular mycorrhizal fungal (AMF) community of the rhizosphere spores andinside the roots of the perennial Polylepisaustralis tree. Three forest types differing in their structural complexitywere chosen at three different sites at the high mountains of centralArgentina. Rhizosphere spores and P.australis roots of four randomly selected trees were isolated from 36 soilsamples; DNA was extracted and the 18S rDNA fragments were amplified bynested?PCR. The products were analyzed by DGGE and the bands were excised forsequencing. Forest disturbance types showed similar rhizosphere spores androot-colonizing communities of AMF. However, differences in AMF compositionbetween rhizosphere spores and within the roots of P. australis were observed. Besides, root banding pattern showedfewer bands than spores. 36 OTUs were defined from 56 DGGE bands successfullysequenced. Members of Glomeraceae, Pacisporaceae, Acaulosporaceae andGigasporaceae were shown in spore samples. Root samples showed only members ofAcaulosporaceae and Gigasporaceae, which might be complementary in terms ofsoil resources exploration. This study presents evidence of a possiblepreference in the AMF? P. australisinteraction.